Tm Tool
v2.0
Checking API…
Sequence (5′→3′)
Clear
Parameters & Mode
Primer
Oligo
Probe
Uses [Primer] in Tm equation
[Strand]
µM
[Monovalent Cation]
mM
[Mg
2+
] total
mM
[dNTP] total
mM
[Complementary strand]
µM
Calculate T
m
Results
Enter a sequence and click
Calculate T
m
Mismatches & Dangling Ends
Reset
Reverse Complement (5′→3′)
Click a base pair to cycle A→T→G→C
■
Match
■
Mismatch
■
Dangling
Dangling End
None
5′ top
3′ top
5′ bottom
3′ bottom
Overhang sequence